Methods for producing enantiomerically pure...

Details Methods for producing enantiomerically pure... Methods for producing enantiomerically pure...

C - Chemistry, Metallurgy

12

N

C12N 15/55 (2006.01) C07K 16/40 (2006.01) C12N 9/78 (2006.01) C12P 7/40 (2006.01) C12P 7/42 (2006.01) C12P 13/04 (2006.01) C12P 41/00 (2006.01)

Patent

CA 2718302

In accordance with the present invention there are provided methods for producing enantiomerically pure a-substituted carboxylic acids, such as, for example, .alpha.-amino acids and .alpha.-hydroxy acids, said method comprising combining an aldehyde or ketone with a cyanide and ammonia or an ammonium salt or an amine, in the presence of a nitrilase or a polypeptide having nitrilase activity which stereoselectively hydrolyzes the amino nitrite or cynohydrin intermediate under conditions sufficient to produce the carboxylic acid.

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