Preservation and storage medium for biological materials

A - Human Necessities – 01 – N

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A01N 1/02 (2006.01)

Patent

CA 2412330

A protectant mixture for use in preserving biological material comprising (1) at least one polyhydroxy compound, where the total amount of polyhydroxy compound in the mixture is from about 5 % to about 60 % by weight of the mixture where the mixture is an aqueous solution and is from about 10 % to about 95 % where the mixture is in solid form, and (2) phosphate ions, where the total amount of phosphate ions in the mixture is such that the molar ratio of phosphate ions to hydroxy groups in the polyhydroxy compound is from about 0.025 to about 0.625; a preservation medium comprising (1) a biological material, (2) at least one polyhydroxy compound, where the total amount of polyhydroxy compound in the medium is from about 5 % to about 60 % by weight of the medium, and (3) phosphate ions, where the total amount of phosphate ions in the mixture is such that the molar ratio of phosphate ions to hydroxy groups in the polyhydroxy compounds is from about 0.025 to about 0.625; methods of preserving the preservation medium; and the resulting preserved biological material composition.

L'invention concerne un mélange protecteur, utile dans la conservation de matériau biologique et comprenant: (1) au moins un composé polyhydroxy présent dans ce mélange, en quantité totale comprise entre environ 5 et environ 60 % en poids du mélange, lequel est une solution aqueuse présente selon une quantité comprise entre environ 10 et environ 95 %, ledit mélange étant sous une forme solide, et (2) des ions phosphate dont la quantité totale présente dans le mélange est telle que le rapport molaire entre les ions phosphate et les groupes hydroxy du composé polyhydroxy est compris entre environ 0,025 et environ 0,625. L'invention concerne également un milieu de conservation comprenant: (1) un matériau biologique, (2) au moins un composé polyhydroxy présent dans ce mélange en quantité totale comprise entre environ 5 et environ 60 % en poids du milieu, et (3) des ions phosphate dont la quantité totale présente dans le mélange est telle que le rapport molaire entre les ions phosphate et les groupes hydroxy du composé polyhydroxy est compris entre environ 0,025 et environ 0,625. L'invention concerne encore des procédés de conservation du milieu de conservation, ainsi que la composition du matériau biologique conservé résultant.

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