Cloned pullulanase

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/56 (2006.01) C07K 14/39 (2006.01) C12N 9/44 (2006.01) C12N 15/67 (2006.01) C12N 15/81 (2006.01)

Patent

CA 2173453

A method is disclosed for the expression of an active pullulanase enzyme in a microorganism host. In one aspect, a DNA construct contains a sequence encoding the pullulanase enzyme, except for the nucleotides necessary to encode the first two amino acids in mature pullulanase and regulating allowing expression of the coding sequence in a microorganism host. An advantageous DNA construct contains regulatory sequences permitting expression of the pullulanase in a yeast cell.

L'invention a pour objet un procédé permettant l'expression d'une enzyme pullulanase active dans un micro-organisme hôte. Selon un aspect de l'invention, un produit de recombinaison d'ADN contient une séquence codant l'enzyme pullulanase, à l'exception des nucléotides nécessaires pour coder les deux premiers acides aminés dans la pullulanase mature, et les séquences de régulation permettant l'expression de la séquence de codage dans un micro-organisme hôte. Un produit de recombinaison d'ADN avantageux contient des séquences de régulation permettant l'expression de la pullulanase dans une cellule de levure.

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