A plasmid for expressing modified recombinant proteins in a...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/70 (2006.01) C07K 14/47 (2006.01) C12N 9/12 (2006.01) C12N 15/12 (2006.01) C12N 15/54 (2006.01) C12N 15/62 (2006.01) C12N 15/64 (2006.01) C12N 15/67 (2006.01)

Patent

CA 2213734

The present invention provides a plasmid containing a promoter sequence, a nucleotide sequence encoding an exogenous protein, and a nucleotide sequence encoding an enzyme capable of modifying the exogenous protein. In a specific embodiment of the invention the encoded exogenous protein is human .beta.- casein and the encoded enzyme is a human kinase capable of phosphorylating recombinant .beta.-casein in a bacterial system.

La présente invention décrit un plasmide contenant une séquence de promoteurs, une séquence nucléotidique codant une protéine exogène et une séquence nucléotidique codant un enzyme capable de modifier cette protéine exogène. Dans un mode de réalisation spécifique, la protéine exogène codée est la caséine .beta. humaine et l'enzyme codé est une kinase humaine capable de réaliser la phosphorylation de la caséine .beta. de recombinaison dans un système bactérien.

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