Flax promoters for manipulating gene expression

Details Flax promoters for manipulating gene expression Flax promoters for manipulating gene expression

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 9/02 (2006.01) C12N 15/53 (2006.01)

Patent

CA 2268745

The invention relates to promoter sequences obtained from flax and useful for modification of flax and other plants for expression of endogenous or foreign genes. The promoters are the SEQ ID NO. 3 and SEQ ID NO. 4 and are obtained from newly illucidated structures of two SAD genes in flax, namely SEQ ID NO. 1 and SEQ ID NO. 2. The promoters have been inserted into cloning plasmids and deposited at the American Type Culture Collection as plasmids pCDC220 and pCDC214 under deposit numbers ATCC 98192 and ATCC 98193, respectively. The promoters may be used in conjunction with genes to modify characteristics of flax and other plants. The invention includes the SAD genes themselves and DNA sequences substantially homologous to SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, and SEQ ID NO. 4, as well as significant parts thereof.

L'invention porte sur des séquences promoteurs obtenues à partir de lin et servant à modifier du lin ou d'autres plantes en vue de l'expression de gènes endogènes ou étrangers. Lesdits promoteurs sont des séquences SEQ ID NO 3 et SEQ ID NO 4 et proviennent de structures nouvellement découvertes de deux gènes SAD du lin, c.-à-d. les séquences SEQ ID NO 1 et SEQ ID NO 2. Les promoteurs ont été placés dans des plasmides de clonage et déposés à la "American Type Culture Collection" sous les appellations pCDC220 et pCDC214 sous les numéros respectifs de dépôt ATCC 98192 et ATCC 98193. Lesdits promoteurs peuvent être utilisés en conjonction avec des gènes pour modifier les caractéristiques du lin et d'autres plantes. L'invention inclut les gènes SAD eux-mêmes et des séquences d'ADN sensiblement homologues des SEQ ID NO 1, SEQ ID NO 2, SEQ ID NO 3 et SEQ ID NO 4, ainsi que leurs parties importantes.

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