Composition for the transfection of higher eucaryotic cells

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/88 (2006.01) A61K 9/127 (2006.01) A61K 31/70 (2006.01) A61K 47/42 (2006.01) A61K 48/00 (2006.01) C12N 15/85 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2246227

The invention concerns a composition for the transfection of higher eucaryotic cells. A complex of a nucleic acid to be expressed in the cell and a cationic lipid present in a suboptimal concentration for transfection contains one or a plurality of membrane-active acid peptides and optionally helper lipid(s). The ratio of the total number of positive charges to the total number of negative charges in the composition is between approximately 0 and approximately 3.

L'invention concerne une composition pour la transfection de cellules eucaryotes supérieures. Un complexe comprenant un acide nucléique à exprimer dans la cellule et un lipide cationique présent dans une concentration sous-optimale pour la transfection, contient un ou plusieurs peptides acides à action membranaire, ainsi qu'éventuellement un(des) lipide(s) auxiliaire(s). Le rapport du nombre total des charges positives au nombre total des charges négatives de la composition est compris entre approximativement 0 et approximativement 3.

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