Complexes of trpc domains and sestd1 domains and methods and...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/47 (2006.01) C07K 14/705 (2006.01)

Patent

CA 2702508

The present invention relates to an isolated complex comprising a first protein comprising or consisting of a classical transient receptor potential channel (TRPC) or a functionally active variant thereof and a second protein comprising or consisting of the first spectrin (Spec 1) domain of SEC14 and spectrin domains 1 (SESTD1), a test system comprising the first protein and the second protein as well as means for detecting interaction of the first and the second protein, a method for screening for a TRPC modulator using the system and the use of the test system for the identification of a TRPC modulator.

L'invention concerne un complexe isolé comprenant une première protéine comprenant ou consistant en un canal potentiel de récepteur transitoire (TRPC) classique ou une variante fonctionnellement active de celui-ci et une seconde protéine composée ou constituée par le premier domaine de spectrine (Spec 1) de SEC14 et les domaines 1 de spectrine (SESTD1), un système d'essai comprenant la première et la seconde protéine ainsi que des moyens de détection de l'interaction de la première et de la seconde protéine, un procédé de criblage d'un modulateur TRPC utilisant le système et l'utilisation du système d'essai pour identifier un modulateur TRPC.

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