Detection and quantification of intracellular pathogens

Details Detection and quantification of intracellular pathogens Detection and quantification of intracellular pathogens

G - Physics

01

N

G01N 33/53 (2006.01) G01N 1/30 (2006.01) G01N 33/571 (2006.01)

Patent

CA 2562241

The present invention provides methods and compositions for detecting bacteria of the Chlamydiaceae family in a biological sample. Methods include, for example, contacting a biological sample with an antibody that specifically binds to a chlamydial antigen displayed on the surface of a chlamydia-infected blood cell; and analyzing the sample using fluorescence microscopy or flow cytometry to detect bound antibody.

La présente invention fournit des procédés et des compositions destinés à détecter des bactéries de la famille Chlamydiaceae dans un échantillon biologique. Les procédés comprennent, par exemple, la mise en contact d'un échantillon biologique avec un anticorps qui se lie spécifiquement à un antigène qui se rapporte aux Chlamydia montrées à la surface d'une cellule sanguine infectée par les Chlamydia, ainsi que l'analyse de l'échantillon en utilisant la microscopie par fluorescence ou la cytométrie par écoulement afin de détecter un anticorps lié.

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