C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C07H 21/02 (2006.01)
Patent
CA 2511381
The present invention relates to compositions comprising probes for the detection of a target polynucleotide, and methods for detecting the amount of a target polynucleotide using the subject composition. In particular, the subject method involves the use of both target-hybridizing probes and non- target-hybridizing probes, where the probes generate a detectable signal (e.g., detectable by FRET) for the target polynucleotide detection.
La présente invention a trait à des compositions comprenant des sondes pour la détection d'un polynucléotide cible, et des procédés pour la détection de la quantité d'un polynucléotide cible mettant en oeuvre la composition de l'invention. En particulier, le procédé de l'invention comprend l'utilisation de sondes d'hybridation à des cibles ainsi que des sondes de non hybridation à des cibles, dans lequel les sondes génèrent un signal détectable (par exemple, détectable par le transfert d'énergie par résonance fluorescente) pour la détection du polynucléotide cible.
Mueller Reinhold Dietrich
Sorge Joseph A.
Sun Gulan
Stratagene California
Torys Llp
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