C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/07 (2010.01)
Patent
CA 2715878
The present invention relates to the field of mammalian cell culture, and provides meth-ods and compositions for cell attachment to, cultiva-tion on and detachment from a solid substrate sur-face containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one em-bodiment of the present invention, the cells are treat-ed with a compound capable of inhibiting Rho ki-nase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity.
L'invention concerne le domaine de la culture de cellules mammaliennes et se rapporte à des procédés et des compositions qui permettent la fixation, la culture et le détachement des cellules sur une surface de substrat solide contenant, pour environ 0,5% de N au moins, un total de O et de N supérieur ou égal à 17,2%, avec un angle de contact d'au moins 13,9 degrés environ, sans couche de cellules nourricières et sans couche adsorbée. Dans un mode de réalisation, on traite les cellules avec un composé capable d'inhiber l'activité Rho kinase. Dans un autre mode de réalisation, on traite les cellules avec un composé capable d'inhiber l'activité Rho.
Brevig Thomas
Fryer Benjamin
Marwood Tina Kristensen
Nelson Shelley
Nielsen Villy
Centocor Ortho Biotech Inc.
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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