Method of cloning animals

Details Method of cloning animals Method of cloning animals

A - Human Necessities

01

K

A01K 67/027 (2006.01) C12N 5/00 (2006.01) C12N 5/06 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2430738

The present invention relates to cloning method of animals. The invention includes cell lines, reconstructed embryos and cloned or transgenic animals. In particular, the invention provides method of cloning animals by combining genome of donor cells at specific stages of the cell cycle without uses of chemical products, with activated enucleated oocyte to thereby obtain reconstructed embryos. The invention further relates to methods of culturing animal cells until confluence in normal conditions avoiding negative genetic mutations induced by chemicals. Also, the invention relates to a method of preparing recipient oocytes before nuclear transfer, and to a culture medium improving the in vitro as well as in vivo development of reconstructed embryos.

L'invention concerne une méthode de clonage d'animaux. Elle porte sur des lignées cellulaires, sur des embryons reconstruits ou sur des animaux transgéniques. L'invention se rapporte, notamment, à une méthode de clonage d'animaux par la combinaison du génome de cellules donatrices à des étapes spécifiques du cycle cellulaire sans utilisation de produits chimiques, à l'aide d'un ovocyte énucléé activé, de sorte que des embryons reconstruits soient obtenus. L'invention se rapporte à des méthodes de culture de cellules animales jusqu'à ce qu'elles soient à confluence dans des conditions normales, ce qui permet d'empêcher les mutations génétiques négatives induites par les produits chimiques. L'invention concerne enfin un procédé de préparation d'ovocytes receveurs avant transfert nucléaire et un milieu de culture améliorant le développement in vitro et in vivo d'embryons reconstruits.

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