Density gradient solutions of metal ion chelate complexes

G - Physics – 01 – N

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G01N 9/30 (2006.01) B01D 17/02 (2006.01) G01N 27/447 (2006.01)

Patent

CA 2439748

Methods of forming a density gradient by applying a centrifugal field to a solution of one or more metal ion chelate complexes are disclosed. The density gradients are self-forming equilibrium gradients and are useful for separating biological particles by ultracentrifugation. Also disclosed are methods of separating biological particles according to their density. Also disclosed are density gradients of lipoprotein particles and one or more metal ion chelate complexes, wherein the lipoprotein particles are partitioned along the density gradient according to their particle density.

Cette invention concerne des procédés permettant de former un gradient de densité en soumettant à un champ de centrifugation une solution d'un ou de plusieurs complexes chélatés d'ions métalliques. Ces gradients de densité, qui sont des gradients de centrifugation à l'équilibre, conviennent bien pour la séparation de particules biologiques par ultracentrifugation. L'invention concerne également des méthodes de séparation de particules biologiques en fonction de leur densité. De plus, l'invention porte sur des gradients de densité de particules de lipoprotéines et d'un ou de plusieurs complexes chélatés d'ions métalliques, les particules de lipoprotéines se séparant le long du gradient de densité en fonction de leur densité respective.

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