Improved immunoglobulin libraries

Details Improved immunoglobulin libraries Improved immunoglobulin libraries

C - Chemistry, Metallurgy

12

N

C12N 7/01 (2006.01) C07K 16/00 (2006.01) C07K 16/12 (2006.01) C12N 1/20 (2006.01) C12N 1/21 (2006.01) C12N 15/00 (2006.01) C12N 15/10 (2006.01) C12N 15/13 (2006.01) C40B 30/04 (2006.01) C40B 40/02 (2006.01) C40B 40/10 (2006.01) C40B 50/06 (2006.01) G01N 33/53 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2671264

Methods and compositions for the screening and isolation of ligand-binding polypeptides, such as antibodies. In some aspects, methods of the invention enable the isolation of intact soluble antibodies comprising a constant domain. Screening methods that employ genetic packages such as bacteria and bacteriophages enable high through-put identification of ligand binding molecules.

L'invention concerne des méthodes et des compositions destinées à cribler et isoler des polypeptides se liant à des ligands, tels que des anticorps. Selon certains aspects, les méthodes dans l'invention permettent d'isoler des anticorps solubles intacts comprenant un domaine constant. Des méthodes de criblage utilisant des empaquetages génétiques comme des bactéries et des bactériophages permettent l'identification de molécules se liant aux ligands avec un rendement élevé.

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