Immunogenic composition based on conditionally live virion...

A - Human Necessities – 61 – K

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A61K 39/12 (2006.01) A61K 39/21 (2006.01) C07K 14/16 (2006.01) C12N 7/00 (2006.01) C12N 15/09 (2006.01) C12N 15/49 (2006.01)

Patent

CA 2632888

A conditionally live virion and method for making the same whereby the viral DNA or RNA is modified so that the virion is incapable of replication unless a protein supplement is added to the expression system. The expression system is either a traditional cell culture or cell free expression system suitable for self assembly of viral particles. Both expression systems require the addition of viral proteins either for replication or assembly of the replication incompetent virion. The conditionally live viron is then used for creating a vaccine with three fold immunogenic properties that are elicited by 1) the whole intact replication incompetent virus; 2) the conditionally live virion temporally resuscitated by addition of protein supplements; and 3) the protein supplement itself acting as a subunit vaccine.

Virion conditionnellement vivant et procédé servant à fabriquer celui-ci consistant à modifier l'ADN ou l'ARN viral de façon à ce que le virion soit incapable de se répliquer sauf si un supplément de protéines est ajouté au système d'expression. Le système d'expression est soit une culture de cellules traditionnelle soit un système d'expression sans cellules convenant pour l'autoassemblage de particules virales. Les deux systèmes d'expression requièrent l'ajout de protéines virales soit pour la réplication soit pour l'assemblage du virion incapable de se répliquer. Le virion conditionnellement vivant est alors utilisé pour créer un vaccin ayant des propriétés immunogènes multipliées par trois qui sont obtenues par 1) le virus incapable de se répliquer intact entier ; 2) le virion conditionnellement vivant provisoirement ressuscité par ajout de suppléments de protéines ; et 3) le supplément de protéines lui-même servant de vaccin sous-unité.

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