Dry chemistry cascade immunoassay and affinity assay

G - Physics – 01 – N

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Details

G01N 33/53 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/543 (2006.01) G01N 33/86 (2006.01)

Patent

CA 2156174

A method is described for performing an affinity assay comprising contacting a sample to be assayed for the presence of an analyte with a dry reagent containing the analyte (hapten, antigen, antibody, receptor, or complementary polynucleotide bound to a reaction cascade initiator, an antibody or other binding pair partner reactive with said analyte, and magnetic particles, to form an assay mixture in a reaction chamber, incubating the assay mixture, applying an oscillating or moving static mgnetic field to the assay mixture, activating the reaction cascade initiator to initiate a reaction cascade, monitoring the response of the magnetic particles to the oscillating or moving static magnetic field to provide a time varying signal, and determining the analyte concentration of the sample by analysis of the time varying signal, as well as a kit for performing the assay and a diagnostic system for performing the assay.

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