C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/75 (2006.01) C12N 15/64 (2006.01) C12N 15/70 (2006.01)
Patent
CA 2059986
CR-8904-A TITLE A METHOD FOR RAPID SELECTION OF EFFICIENT SECRETION VECTORS ABSTRACT A method for rapidly selecting a vector which maximizes secretion of a mature heterologous protein by bacteria as described. The method involves construction of several recombinant vectors having signal sequence and mature protein sequence from different sources. The promoter and ribosome binding site might also be selected from different sources from those of the signal sequence or mature protein sequence. Rapid construction of recombinant vectors is made possible by selection of compatible restriction sites on the 3' end of the signal sequence and the 5' end of the mature protein sequence. RTG/bjm
Bennett Jones Llp
E.i. Du Pont de Nemours And Company
LandOfFree
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