Mammalian cancer cell and transgenic mammal carrying human...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 5/16 (2006.01) A01K 67/027 (2006.01) C07K 14/82 (2006.01) C12N 5/06 (2006.01) C12N 15/85 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2433176

The present invention provides a mammalian cell transfected with an expression vector comprising a human protooncogene which has the nucleotide sequence of SEQ ID NO: 1; a mammalian embryo carrying a nucleic acid construct comprising the human protooncogene; a transgenic cancered mammal, which is derived from the mammalian embryo; and a kit for diagnosing a cancer selected from the group consisting of breast, kidney, ovary and stomach cancers, which comprises a probe having the nucleotide sequence complementary to mRNA transcribed from the human protooncogene or a portion of the mRNA; or an antibody binding specifically to a protein translated from the mRNA or a portion of the protein.

La présente invention concerne une cellule mammifère transfectée par un vecteur d'expression comportant un protooncogène humain présentant la séquence nucléotidique SEQ ID NO : 1 ; un embryon mammifère portant une construction d'acides nucléiques comportant le protooncogène humain ; un mammifère cancéreux transgénique dérivé de l'embryon mammifère ; et, un ensemble de diagnostic du cancer, notamment du cancer du sein, du rein, de l'ovaire, et de l'estomac, ledit ensemble comportant une sonde présentant la séquence nucléotidique complémentaire de l'ARNm transcrit à partir du protooncogène humain ou d'une partie de cet ARNm, ou un anticorps se liant spécifiquement à une protéine traduite à partir de l'ARNm ou à une partie de la protéine.

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