Borrelia burgdorferi spirochete specific nucleic acid probes

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2155756

An oligonucleotide agent for use in revealing at least one Borrelia burgdorferi strain. Sequences common to all Borrelia burgdorferi strain groups are provided for use as primers in polymerase chain reactions, as capture probes in a solid substrate hybridization test in which target DNA is "sandwiched" between the capture probe and a revelatory probe, or as revelatory probes in said solid substrate hybridization test. Sequences specific to each of the Bb strain groups are also provided for use as primers in polymerase chain reactions, or as revelatory or capture probes in the above-mentioned solid substrate hybridization test.

L'invention concerne un agent oligonucléotidique utilisable pour la mise en évidence d'au moins une souche de Borrelia burgdorferi. On propose des séquences communes à tous les groupes de souches de Borrelia burgdorferi, utilisables soit comme amorces su cours de réactions d'amplification génétique, soit comme sondes de capture dans un test d'hybridation sur support solide où l'ADN cible est pris en "sandwich" entre cette sonde de capture et une sonde de révélation, soit comme sondes de révélation dans ce test d'hybridation sur support solide. On propose également des séquences spécifiques de chacun des groupes de souches de Bd, utilisables comme amorces au cours de réactions d'amplification génétique ou comme sondes de révélation ou de capture dans le test d'hybridation sur support solide évoqué ci-dessus.

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