Isolation of enzymes

Details Isolation of enzymes Isolation of enzymes

C - Chemistry, Metallurgy

12

N

C12N 9/00 (2006.01) C07K 14/47 (2006.01) C07K 14/72 (2006.01) C12N 9/10 (2006.01) C12N 9/12 (2006.01) C12N 9/96 (2006.01) C12N 15/10 (2006.01) C12N 15/52 (2006.01)

Patent

CA 2250922

The present invention relates to a method of isolating an enzyme having a desired chemical or biological activity, the method comprising linking the enzyme to a substrate for the enzyme, reacting the enzyme and substrate such that a product is produced which remains linked to the enzyme and isolating the enzyme by selectively isolating the product linked to the enzyme.

L'invention concerne un procédé permettant d'isoler une enzyme ayant une activité chimique ou biologique désirée. Selon ce procédé, on lie l'enzyme à un substrat qui lui est destiné, on fait réagir l'enzyme et le substrat de façon à obtenir un produit qui reste lié à l'enzyme, et on isole l'enzyme en isolant sélectivement le produit lié à l'enzyme.

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