C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/62 (2006.01) C07K 14/435 (2006.01) C07K 16/18 (2006.01) C07K 16/40 (2006.01) C12N 9/02 (2006.01) C12N 9/96 (2006.01) C12N 15/12 (2006.01) C12N 15/53 (2006.01) C12N 15/65 (2006.01) C12Q 1/66 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2267068
A fusion gene is provided comprising the cDNA of Renilla luciferase and the cDNA of the "humanized" Aequorea green fluorescent protein. The fusion gene was used to produce a novel protein, the "Renilla-GFP fusion protein", which displayed both the luciferase activity of Renilla luciferase, and the green fluorescence of GFP. The Renilla-GFP fusion gene is useful as a double marker for monitoring gene expression quantitatively in UV light and by enzyme activity.
L'invention concerne un gène de fusion qui contient l'ADNc de luciférase Renilla et l'ADNc de la protéine fluorescente verte (GFP) d'Aequorea "humanisée". Le gène de fusion a été utilisé pour produire une protéine nouvelle, la "protéine de fusion Renilla-GFP", qui présente à la fois l'activité luciférase de la luciférase Renilla et la fluorescence verte de la GFP. Le gène de fusion Renilla-GFP est utilisé comme marqueur double servant à surveiller l'expression génique de manière quantitative en lumière UV et par activité enzymatique.
Szalay Aladar A.
Wang Gefu
Wang Yubao
Gowling Lafleur Henderson Llp
Loma Linda University
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