Propagation and/or derivation of embryonic stem cells

Details Propagation and/or derivation of embryonic stem cells Propagation and/or derivation of embryonic stem cells

C - Chemistry, Metallurgy

12

N

C12N 5/0735 (2010.01)

Patent

CA 2342290

Embryonic stem (ES) cells are cultured in the presence of a compound which selectively inhibits propagation or survival of cells other than ES cells. The ES cells have not been genetically altered. Instead, the compound inhibits a signalling pathway which is essential for propagation of differentiated cells but is not essential for propagation of ES cells - hence ES cells are selectively maintained in the culture.

Des cellules souches embryonnaires (cellules ES) sont cultivées en présence d'un composé qui inhibe sélectivement la prolifération ou la survie des cellules autres que lesdites cellules ES. Ces cellules ES ne sont pas génétiquement modifiées. Le composé inhibe une voie de signalisation qui est essentielle à la prolifération des cellules différenciées, mais pas à celle des cellules ES, ce qui permet le maintien sélectif de ces dernières en culture.

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