Mutant 1,3-propanediol dehydrogenase

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/53 (2006.01) C12N 1/20 (2006.01) C12N 1/21 (2006.01) C12N 9/04 (2006.01) C12P 7/18 (2006.01)

Patent

CA 2373312

The present invention relates to mutant 1,3-propanediol dehydrogenase and a novel microorganism that is capable of growing in concentrations of at least 105 g/l 1,3-propanediol, levels normally toxic to wild-type microorganisms. The present invention also provides expression vectors and host cells comprising the mutant 1,3-propanediol dehydrogenase as well as methods for producing 1,3-propanediol comprising the use of cells comprising the mutant 1,3-propanediol dehydrogenase.

L'invention concerne la 1,3-propanediol déshydrogénase mutante et un nouveau microorganisme capable de se développer dans des concentrations d'au moins 105g/l de 1,3-propanediol, des niveaux habituellement toxiques pour les microorganismes de type sauvage. L'invention concerne également des vecteurs d'expression et des cellules hôtes renfermant la 1,3-propanediol déshydrogénase mutante, ainsi que des méthodes de préparation du 1,3-propanediol consistant à utiliser les cellules renfermant la 1,3-propanediol déshydrogénase mutante.

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