Genetically engineered primary oligodendrocytes for...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/10 (2006.01) A61K 35/30 (2006.01) C12N 5/06 (2006.01) C12N 15/867 (2006.01) A61K 35/12 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2247912

The present invention pertains to a method for transplanting genetically engineered primary cells into the central nervous system of a patient. The method comprises the steps of (a) isolating primary oligodendrocyte progenitor cells from the brain of a patient; (b) expanding the cells in vitro with mitogens; (c) genetically engineering the cells by introducing transgenes through an eukaryotic expression vector for DNA-mediated gene transfer into the cells; and (d) transplanting the genetically engineered primary cells into the brain of the patient.

Cette invention concerne un procédé permettant de transplanter dans le système nerveux central d'un patient, des cellules primaires obtenues par génie génétique. Ce procédé comprend les étapes suivantes: (a) isoler des cellules progénitrices d'oligodendrocytes primaires à partir du cerveau du patient; (b) développer ces cellules in vitro avec des mitogènes; (c) modifier les cellules par génie génétique en introduisant des transgènes par l'intermédiaire d'un vecteur d'expression eucaryote pour le transfert de gènes par ADN dans les cellules; et (d) transplanter les cellules primaires obtenues par génie génétique dans le cerveau du patient.

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