Very low protein nutrient medium for cell culture

C - Chemistry – Metallurgy – 12 – N

Patent

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195/43

C12N 5/02 (2006.01) C12N 5/00 (2006.01)

Patent

CA 2001550

ABSTRACT OF THE DISCLOSURE This nutrient medium is very effective for the serum-free or serum-protein-free culture of various animal cells, in both high and low density culture. Serum proteins have been replaced with non-protein- based cell growth enhancers and a non-serum derived protein supplement. The non-protein growth enhancer is a modified or derivatized polyurethane prepolymer or polymer and preferably is a sulfhydryl derivative of polyurethane. The protein supplement may be insulin, an insulin analog or an insulin-like growth factor.

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