C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/12 (2006.01) A61K 48/00 (2006.01) C07H 21/04 (2006.01) C07K 14/47 (2006.01) C07K 14/705 (2006.01) C12N 15/00 (2006.01) C12N 15/08 (2006.01) C12N 15/09 (2006.01) C12N 15/63 (2006.01) C12N 15/85 (2006.01)
Patent
CA 2346430
The present invention provides tangible means and methods for stimulation of angiogenesis via enhanced endothelial expression of core proteins having a syndecan-4 cytoplasmic region intracellularly. The tangible means include a prepared DNA sequence fragment having separate and individual DNA sequenced portions coding for an heparan sulfate binding extracellular domain, a central transmembrane domain, and a cytoplasmic domain coding for the syndecan-4 polypeptide. The prepared DNA sequence unitary fragment may be delivered to endothelial cells in-situ, both under in-vivo and/or in-vitro conditions, using suitable expression vectors including plasmids and viruses. The resulting transfected endothelial cells overexpress heparan sulfate binding, core proteins; and the resulting overexpression of these proteoglycan entities causes stimultation of angiogenesis in-situ.
L'invention concerne des moyens et des procédés tangibles pour la stimulation d'angiogenèse par expression endothéliale accrue de protéines coeurs présentant une région intracellulaire syndécane-4 cytoplasmique. Les moyens tangibles comprennent un fragment de séquence d'ADN préparé ayant des portions séquencées d'ADN séparées et individuelles codant pour un domaine extracellulaire liant un sulfate d'héparane, un domaine transmembrane central et un domaine cytoplasmique codant pour le syndécane-4 polypeptide. Le fragment unitaire de séquence d'ADN préparé peut être fourni aux cellules endothéliales in situ, dans des conditions in vivo et/ou in vitro, en utilisant des vecteurs d'expression appropriés y compris des plasmides et des virus. Les cellules endothéliales transfectées résultantes surexpriment la liaison sulfate d'héparane des protéines coeurs, et la surexpression résultante de ces entités de protéoglycane provoque la stimulation de l'angiogenèse in situ.
Horowitz Arie
Simons Michael
Volk Rudiger
Beth Israel Deaconess Medical Center
Smart & Biggar
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