Polynucleotide amplification method

Details Polynucleotide amplification method Polynucleotide amplification method

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2378408

Methods useful for improving results obtained with enzyme-based polynucleotide amplification reactions. More particularly, the invented methods are useful for: (1) promoting amplification of template-specific products such that the amount of amplicon produced reflects the pre-amplification amount of analyte, even in reactions primed with low levels of analyte polynucleotide; (2) facilitating biological specimen processing such that the amount of amplicon produced in subsequent amplification reactions will be substantially independent of the efficiency of analyte polynucleotide isolation from the specimen; and (3) controlling the amount of analyte amplicon produced in the amplification reaction.

L'invention concerne des méthodes d'amélioration de résultats obtenus dans des réactions d'amplification de polynucléotides à base d'enzymes. Plus particulièrement, les méthodes selon l'invention s'utilisent : (1) pour favoriser l'amplification de produits à matrice spécifique de sorte que la quantité d'amplicon produite reflète le degré de pré-amplification de substances à analyser, même dans des réactions amorcées avec de faibles niveaux de polynucléotides de substances à analyser ; (2) pour faciliter le traitement d'échantillons biologiques de sorte que la quantité d'amplicon produite dans des réactions d'amplification ultérieures soit sensiblement indépendante de l'efficacité de l'isolement de polynucléotides de substances à analyser de l'échantillon ; et (3) pour réguler la quantité d'amplicon de substances à analyser produite dans la réaction d'amplification.

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