Improvements in and relating to identification

Details Improvements in and relating to identification Improvements in and relating to identification

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2223110

DNA profiling provides an extremely useful tool in a wide variety of applications including anthropological studies, paternity investigations and in consideration of forensic evidence. The present invention provides improved primers, primer pairs and loci selections for giving increased discriminating power in DNA profiling techniques making use of short tandem repeats (STR) or micro satellite loci. A primer pair mixture including sequences:- pair a) TGC AGA TCT TGG GAC TTC TC AAA AAG AGT GTG GGC AAC TG pair b) CCA GTG GAT TTG GAA ACA GA ACC TAG CAT GGT ACC TGC AG pair c) ACT GCA GTC CAA TCT GGG T ATG AAA TCA ACA GAG GCT TG pair d) GCA ACA TTA GGT ACA TAC AAG AAA ATA ATC AGG GTG CTG TGT pair e) TAC ATC CAA ATT AGG TAG GTA GAC AA TGT TGA GAC ACA GCA GTA GCA T pair f) CTG TCT CAG ACC ATA CCT AAA TCT CT TCT TAG TGG GGA AGG GAT CCT AGT TAT CAC pair g) GTG GGC TGA AAA GCT CCC GAT TAT GTG ATT CCC ATT GGC CTG TTC CTC is provided.

L'établissement du profil d'ADN représente un outil extrêmement précieux dans toute une gamme d'applications, dont les études anthropologiques, les cas de recherche de paternité et dans l'examen des preuves judiciaires. La présente invention fournit des amorces améliorées, des paires d'amorces et des sélections de loci pour donner un pouvoir discriminant plus élevé dans les techniques d'établissement du profil d'ADN faisant appel aux courtes séquences répétées en tandem (CSRT) ou des micro-loci satellites. Un mélange de paires d'amorces incluant des séquences : - paire a) TGC AGA TCT TGG GAC TTC TC AAA AAG AGT GTG GGC AAC TG paire b) CCA GTC GAT TTG GAA ACA GA AAC TAG CAT GGT ACC TGC AG paire c) ACT GCA GTC CAA TCT GGG T ATG AAA TCA ACA GAG GCT TG paire d) GCA ACA TTA GGT ACA TAC AAG AAA ATA ATC AGG GTG CTG TGT paire e) TAC ATG CAA ATT AGG TAG GTA GAC AA TGT TGA GAC ACA GCA GTA GCA T paire f) CTG TCT CAG ACC ATA CCT AAA TCT CT TCT TAG TGG GGA AGG GAT CCT AGT TAT CAC paire g) GTG GGC TGA AAA GCT CCC GAT TAT GTG ATT CCC ATT GGC CTG TTC CTC est fourni.

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