Serotype-specific probes for listeria monocytogenes

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/31 (2006.01) C07K 14/195 (2006.01) C07K 16/12 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2333856

Recombinant nucleic acids comprising region(s) of Listeria monocytogenes genome that are unique to an individual serotype and genomic cluster are provided. Also provided are oligonucleotide probes and primers derived from the recombinant nucleic acid sequences and methods for their use in the detection and identification of serovar 4 and genomic IIB strains.

La présente invention concerne des acides nucléiques de recombinaison comprenant une ou plusieurs régions du génome de la Listeria monocytogènes particulières à un seul sérotype et agrégat génomique. Cette invention concerne par ailleurs des sondes et des amorces oligonucléotides obtenues à partir des séquences d'acide nucléique de recombinaison, ainsi que leurs procédés d'utilisation pour la détection et l'identification des souches IBB d'agrégat génomique et de sérovar 4.

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