Regulation of cardiac contractility and heart failure...

A - Human Necessities – 01 – K

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A01K 67/027 (2006.01)

Patent

CA 2538999

The methods and compositions of the present invention find use in altering expression of PKC.alpha. in transgenic animals. The compositions of the invention include isolated transgenic animal cells, transgenic tissue, transgenic animals, and transgenic mice. The transgenic animals of the invention exhibit altered PKC.alpha. activity. The methods allow generation of transgenic animals with altered expression of PKC.alpha.. The invention allows modulation of cardiac contractility. In particular, the invention provides a method for altering the susceptibility of a transgenic animal to cardiomyopathy. A transgenic animal of the invention finds use in identifying anti-cardiomyopathic compounds.

La présente invention a trait à des procédés et des compositions utiles dans la modification de l'expression de la protéine kinase C-alpha (PKC.alpha.) chez des animaux transgéniques. Les compositions de l'invention comprennent des cellules animales transgéniques isolées, du tissu transgénique, des animaux transgéniques, et des souris transgénique. Les animaux transgéniques de l'invention présentent une activité de PKC.alpha. modifiée. Les procédés permettent la génération d'animaux transgéniques à expression modifiée de PKC.alpha.. L'invention permet la modulation de la contractilité cardiaque. En particulier, l'invention fournit un procédé pour la modification de la susceptibilité d'un animal transgénique à la cardiomyopathie. Un animal transgénique de l'invention trouve une application dans l'identification de composés contre la cardiomyopathie.

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