Methods and compositions for targeted integration

Details Methods and compositions for targeted integration Methods and compositions for targeted integration

C - Chemistry, Metallurgy

12

N

C12N 15/867 (2006.01)

Patent

CA 2703045

Disclosed herein are methods and compositions for targeted integration of one or more copies of a sequence of interest using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain and integrase defective lentiviral donor constructs.

L'invention porte sur des procédés et sur des compositions pour une intégration ciblée d'une ou plusieurs copies d'une séquence d'intérêt à l'aide de nucléases à doigt de zinc (ZFN) comprenant une protéine à doigt de zinc et un domaine de clivage ou un demi-domaine de clivage et des produits de construction de donneurs lentiviraux défectueux en intégrase.

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