Composition for isolating apamin receptors, apamin binding...

Details Composition for isolating apamin receptors, apamin binding... Composition for isolating apamin receptors, apamin binding...

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) A61K 39/00 (2006.01) C07H 21/00 (2006.01) C07K 1/22 (2006.01) C07K 14/705 (2006.01) C07K 16/28 (2006.01) C07K 17/10 (2006.01) C12N 5/10 (2006.01) C12P 21/08 (2006.01) C12Q 1/02 (2006.01) G01N 33/566 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2101468

COMPOSITION FOR ISOLATING APAMIN RECEPTORS, APAMIN BINDING PROTEIN, AND USES THEREOF Abstract of the Disclosure The present invention relates to a compositon of matter useful as an affinity matrix for isolating an apamin receptor from cellular material, comprising a derivatized agerose matrix having a free amino group covalently coupled to a free carboxyl group of apamin. It additionally relates to a protein, isolated from a vertebrate tissue sample, of approximately 80 KDa which specifically binds apamin, and to an approximately 55 KDa presumed degradation product of said protein, as well as antibodies which bind to said protein or to the presumed degradation product. The invention further relates to an isolated nucleic acid fragment, comprising a nucleic acid sequence encoding an apamin receptor protein, or a biologically active fragment thereof; and to host cells comprising said nucleic acid fragment.

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