Method for transformation of agaricus bisporus

Details Method for transformation of agaricus bisporus Method for transformation of agaricus bisporus

C - Chemistry, Metallurgy

12

N

C12N 15/63 (2006.01) C12N 1/14 (2006.01) C12N 15/11 (2006.01) C12N 15/80 (2006.01)

Patent

CA 2452183

Applicants have devised a highly effective, convenient, and expeditious genetic transformation system for filamentous fungi, such as Agaricus bisporus. The preferred method uses an Agrobacterium-mediated transformation protocol. The critical features of this protocol include co-cultivation of the bacterium with fruit body tissue instead of spores. In a preferred embodiment, even higher transformation efficiencies were observed with the use of a homologous promoter in the polynucleotide expression construct in order to drive gene expression.

L'invention concerne un système de transformation génétique de rendement élevé et d'utilisation aisée et rapide, de champignons filamenteux tels que Agaricus bisporus. Le procédé préféré fait intervenir un protocole de transformation induit par Agrobacterium. Ledit protocole fait notamment intervenir la co-culture de la bactérie avec des tissus de carpophores au lieu de spores. Dans un mode de réalisation préféré, des rendements de transformation plus élevés peuvent être obtenus avec l'utilisation d'un promoteur homologue dans la construction d'expression polynucléotidique de manière à commander l'expression génétique.

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