Compositions and methods for the discovery and selection of...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) A61K 31/7088 (2006.01) A61K 45/00 (2006.01) A61K 48/00 (2006.01) A61P 35/00 (2006.01) A61P 43/00 (2006.01) C07H 21/04 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/00 (2006.01) C12N 15/09 (2006.01) C12N 15/11 (2006.01) C12N 15/74 (2006.01) C12Q 1/02 (2006.01) G01N 33/15 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2426896

Methods and vectors are disclosed for discovering intracellular regulatory pathways utilized by specific stimulatory agents. Suitable stimulatory agents include cytokines, chemical agents, and antibodies. Cell lines and regulatory factors are provided for screening libraries of drug candidates to identify potential therapeutic agents. Methods and compositions are also provided for identifying genes which are necessary for, or capable of, up regulating or down regulating the targeted genomic loci in the selected cells.

L'invention concerne des méthodes et des vecteurs permettant de découvrir des mécanismes de régulation intracellulaires utilisés par des agents stimulants spécifiques. Les agents stimulants appropriés comprennent des cytokines, des agents chimiques et des anticorps. Les lignées cellulaires et les facteurs de régulation servent à cribler des banques de médicaments candidats et à identifier ainsi les agents thérapeutiques potentiels. Par ailleurs, l'invention concerne des méthodes et des compositions d'identification de gènes essentiels/capables d'induire la régulation positive ou négative des locus génomiques cibles dans les cellules sélectionnées.

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