Method for producing gamma-carboxylated proteins

Details Method for producing gamma-carboxylated proteins Method for producing gamma-carboxylated proteins

C - Chemistry, Metallurgy

12

N

C12N 9/64 (2006.01)

Patent

CA 2541764

The present invention relates to methods and tools for producing large quantities of gamma-carboxylated protein comprising: (i) culturing a cell adapted to express a protein which requires gamma-carboxylation and .gamma.- glutamyl carboxylase in a ratio of at least 10:1, under conditions suitable for expression of both proteins, and (ii) isolating gamma-carboxylated protein.

La présente invention concerne des procédés et des outils de fabrication de grandes quantités de protéines gamma-carboxylées. Les procédés selon l'invention consistent (i) à cultiver une cellule capable d'exprimer une protéine nécessitant une gamma-carboxylation et une ?-glutamyl carboxylase dans un rapport d'au moins 10:1, dans des conditions adaptées à l'expression des deux protéines; et (ii) à isoler la protéine gamma-carboxylée.

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