Dehydroquinate dehydrase/shikimate dehydrogenase as a...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/00 (2006.01) C12N 9/04 (2006.01) C12N 9/88 (2006.01) C12N 15/82 (2006.01) C12Q 1/32 (2006.01) C12Q 1/527 (2006.01)

Patent

CA 2415382

The invention relates to the use of a nucleic acid sequence coding for a polypeptide exhibiting dehydroquinate dehydrase/shikimate dehydrogenase activity for producing a test system for discovering the inhibitors of dehydroquinate dehydrase/shikimate dehydrogenase. For the first time, it has been shown, with the aid of the antisense technology, that dehydroquinate dehydrase/shikimate dehydrogenase constitutes a herbicide target. The invention further relates to a method for producing transgenic plants containing a nucleic acid sequence coding for a polypeptide containing dehydroquinate dehydrase/shikimate dehydrogenase (E.C. 4.2.1.10/E.C. 1.1.1.25) activity, characterised by an increased biomass production or/and an increased content of aromatized amino acids compared to a non-transgenic plant.

L'invention concerne l'utilisation de séquences d'acides nucléiques, codant un polypeptide présentant l'activité de la déshydroquinate déshydratase/shikimate déshydrogénase, pour produire un système de test servant à trouver des inhibiteurs de la déshydroquinate déshydratase/shikimate déshydrogénase. Pour la première fois, on a montré au moyen de la technologie antisens que la déshydroquinate déshydratase/shikimate déshydrogénase constitue une cible d'herbicide. L'invention concerne en outre un procédé de production de plantes transgéniques qui contiennent une séquence d'acides nucléiques codant un polypeptide présentant l'activité de la déshydroquinate déshydratase/shikimate déshydrogénase (E.C. 4.2.1.10/E.C. 1.1.1.25) et qui sont caractérisées par une production de biomasse accrue et/ou une teneur plus élevée en acides aminés aromatiques, par rapport à une plante non transgénique.

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