C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/11 (2006.01) C12N 9/64 (2006.01) C12N 15/62 (2006.01) C12N 15/85 (2006.01)
Patent
CA 2279552
The present invention provides a genetic construct providing preferential expression in specific tissue of a coding sequence. The construct includes the following elements (1): a steroid responsive promoter from a tissue specific gene, (2) a coding sequence, and (3) an SV40 enhancer. The elements are arranged as follows: the steroid responsive promoter from the tissue specific gene being upstream of the coding sequence and the SV40 enhancer being 3' of the coding sequence, or the SV40 enhancer being upstream of the coding sequence and the steroid responsive promoter from the tissue specific gene being positioned between the SV40 enhancer and the coding sequence, or the steroid responsive promoter from the tissue specific gene being upstream of the coding sequence and the SV40 enhancer being positioned within an intron within the coding sequence. The promoter has a lowered level of steroid responsiveness in the construct than in its native state.
L'invention concerne une construction génétique permettant l'expression préférentielle, dans un tissu spécifique, d'une séquence codante. La construction comprend les éléments suivants: (1) un promoteur sensible aux stéroïdes, provenant d'un gène spécifique de tissu, (2) une séquence codante, et (3) un activateur SV40. Ces éléments sont disposés de la façon suivante: le promoteur sensible aux stéroïdes provenant du gène spécifique de tissu est en amont de la séquence codante et l'activateur SV40 en position 3' de la séquence codante; l'activateur est en amont de la séquence codante, et le promoteur entre l'activateur et la séquence codante; ou bien le promoteur est en amont de la séquence codante et l'activateur à l'intérieur d'un intron de la séquence codante. La sensibilité du promoteur aux stéroïdes est plus faible dans la construction qu'à l'état natif.
Commonwealth Scientific And Industrial Research Organisation
Swabey Ogilvy Renault
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