Method of harvesting rare cells from blood products

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/078 (2010.01) C12Q 1/04 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2286156

The present invention provides methods of harvesting rare cells from blood products and/or obtaining products of the rare cells. The method includes contacting a blood product containing rare cells with a porous medium, and selectively retaining rare cells of interest on the porous medium. The porous medium can be contacted with an elution fluid wherein a population of the rare cells is eluted from the porous medium. Rare cells selectively retained on the porous medium can be cultured on the porous medium, and products of the rare cells can be obtained.

Cette invention se rapporte à des procédés servant à prélever des cellules rares dans des produits sanguins et/ou à obtenir des produits de ces cellules rares. Ce procédé consiste à mettre un produit sanguin contenant des cellules rares en contact avec un support poreux, et à retenir sélectivement les cellules rares d'intérêt sur le support poreux. Le support poreux peut être mis en contact avec un fluide d'élution, dans lequel une population desdites cellules rares est séparée par élution du support poreux. Les cellules rares retenues sélectivement sur le support poreux peuvent être cultivées sur celui-ci et des produits de ces cellules rares peuvent être obtenus.

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