Nitrate reductases from porphyra, compositions and methods...

Details Nitrate reductases from porphyra, compositions and methods... Nitrate reductases from porphyra, compositions and methods...

C - Chemistry, Metallurgy

12

N

C12N 9/02 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2690797

The NR enzymes described herein were discovered in the red algae of Porphyra perforata (PpNR) and Porphyra yezoensis (PyNR). The present invention provides methods and compositions relating to altering NR activity, nitrogen utilization and/or uptake in plants. The invention relates to a method for the production of plants with maintained or increased yield under low nitrogen fertility. The invention provides isolated nitrate reductase (NR) nucleic acids and their encoded proteins. The invention further provides recombinant expression cassettes, host cells, and transgenic plants. Plants transformed with nucleotide sequences encoding the NR enzyme show improved properties, for example, increased yield and growth.

Les enzymes NR de la présente invention ont été découverts dans les algues rouges de la Porphyra perforata (PpNR) et de la Porphyra yezoensis (PyNR). La présente invention concerne des procédés et des compositions portant sur l'altération de l'activité NR, l'utilisation et/ou l'absorption d'azote dans les plantes. L'invention porte sur un procédé de production de plantes présentant un rendement identique ou accru dans des conditions de faible fertilité azotée. L'invention a trait à des acides nucléiques isolés de nitrate réductase (NR) et les protéines qu'ils codent. L'invention se rapporte en outre à des cassettes d'expression de recombinaison, des cellules hôtes et des plantes transgéniques. Les plantes transformées, sous l'effet du codage de l'enzyme NR par les séquences de nucléotides, présentent des propriétés améliorées tels un rendement et une croissance accrus.

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