Method of detecting a pathogen using a virus

Details Method of detecting a pathogen using a virus Method of detecting a pathogen using a virus

G - Physics

01

N

G01N 33/569 (2006.01) C12Q 1/70 (2006.01) G01N 33/554 (2006.01)

Patent

CA 2130072

A bacteriophage linked to an enzyme can replace an antibody in a system for detecting the presence of a bacteria in a sample. Specifically Brucella abortus (a pathogen which causes brucellosis in cattle) can be detected using Brucella bacteriophage for the virus, urease for the enzyme linked to the bacteriophage, m-maleimidobenzoyl-N-hydrosysuccimide ester as a coupling reagent, sera from mice immunized with Brucella bacteriophage for a detector antibody, urease conjugated to anti-mouse sheep antibody for an indicator, and urea with bromcresol purple as the substrate. The materials can be used in indirect (sandwich) or direct enzyme-linked viral assays (ELVirA).

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