Altering carotenoid profiles in plants

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01) A01H 3/00 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01) C12N 15/31 (2006.01) C12N 15/53 (2006.01)

Patent

CA 2678762

A method for altering the level of one or more carotenoids in a plant or a tissue within the plant is provided. Method comprises introducing a nucleic acid sequence comprising a regulatory region operatively associated with a silencing nucleotide sequence that reduces or eliminates the expression of a lycopene epsilon cyclase into the plant, and expressing the silencing nucleotide sequence. Expression of the sequence reduces the level of the lycopene epsilon cyclase in the plant or within a tissue of the plant, and results in altering one or more carotenoids.

L'invention concerne une méthode de modification du niveau d'un ou de plusieurs caroténoïdes d'une plante ou d'un tissu à l'intérieur de la plante. La méthode consiste à introduire une séquence d'acides nucléiques comprenant une région régulatrice fonctionnellement associée à une séquence nucléotidique de silençage qui réduit ou élimine l'expression d'une lycopène epsilon cyclase de la plante, et à exprimer la séquence nucléotidique de silençage. L'expression de la séquence réduit le niveau de la lycopène epsilon cyclase de la plante ou à l'intérieur du tissu d'une plante, et entraîne une modification d'un ou de plusieurs caroténoïdes.

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