C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C12Q 1/70 (2006.01)
Patent
CA 2136831
Nucleic acid analogues of the kind described in PCT/EP92/01220 (PNA's) which hybridise strongly to nucleic acids are used to inhibit nucleic acid amplification procedures such as PCR. False positives in subsequent PCR assays are prevented by hybridising a PNA to PCR amplification products. Assays capable of discriminating between single base pair mutants are con- ducted by using a PNA hybridising to one of the two allelic forms to inhibit a PCR amplification of that form selectively. Asym- metric PCR amplifications are carried out by starting a PCR symmetricaly using like quantities of forward and reverse primers and once the amplification is established disabling one primer by hybridising a PNA thereto.
Berg Rolf H.
Burchardt Ole
Egholm Michael
Nielsen Peter E.
Stanley Christopher John
Berg Rolf H.
Buchardt Dorte
Egholm Michael
Nielsen Peter E.
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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