In situ pcr amplification system

Details In situ pcr amplification system In situ pcr amplification system

C - Chemistry, Metallurgy

12

M

C12M 1/40 (2006.01) B01J 19/00 (2006.01) B01L 3/00 (2006.01) B01L 7/00 (2006.01) C12Q 1/68 (2006.01) G01N 1/31 (2006.01)

Patent

CA 2106360

Abstract of the Disclosure A complete in situ PCR system for amplification of nucleic acids contained in a prepared cell or tissue sample. The containment system for the PCR sample comprises a glass microscope slide, a specimen sample containing the target nucleic acid sequence mounted on the slide, a flexible plastic cover over the sample, and a retaining assembly fastened to the slide and to the cover to retain and seal a reaction mixture against the sample during thermal cycling. The retaining assembly includes a rigid ring on a rim portion of the cover, a cross beam having spaced parallel rails joined by opposite flat ends, and a pair of clips which are pressed over the ends and opposite sides of the slide to fasten the cross beam and cover to the slide.

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