Translational enhancer dna

Details Translational enhancer dna Translational enhancer dna

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) C07K 14/47 (2006.01) C12N 15/62 (2006.01) C12N 15/67 (2006.01)

Patent

CA 2174863

The gene which expresses the light (.beta.-)chain of kinesin contains at its 5'-end a region of DNA of high secondary structure which provides a strong enhancer of translation in the human kinesin gene it forms a double hairpin loop. The secondary structure, optionally with flanking sequence, is claimed as isolated DNA. Constructs in which it serves is an enhancer for expression of foreign DNA are also claimed.

Le gène qui exprime la chaîne (.beta.-) légère de kinésine contient à son extrémité 5' une région d'ADN de structure secondaire élevée améliorant considérablement la traduction. Dans le gène humain de la kinésine, cette région d'ADN forme une double boucle en épingle à cheveux. La structure secondaire éventuellement bordée d'une séquence est revendiquée comme étant de l'ADN isolé. L'invention se rapporte également à des produits de recombinaison dans lesquels ladite région est un améliorant d'expression d'ADN étranger.

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