Use of a virus dna as promoter

Details Use of a virus dna as promoter Use of a virus dna as promoter

C - Chemistry, Metallurgy

12

N

C12N 15/70 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 15/74 (2006.01) C12N 15/80 (2006.01) C12N 15/81 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2296392

The invention relates to the characterization and use of powerful viral promoters for expressing genes in bacteria and fungi, especially yeasts. The invention is based on a surprising discovery, namely that CFDV-DNA (DNA of the coconut foliar decay virus) and fragments of CFDV-DNA contain a region active as promoter even in bacteria and fungi, especially yeasts, although they originate from a virus which infects monocotyledonous plants. The activity of these promoters in E. coli is markedly greater than that of the CaMV 35S promoter which is also active in bacteria.

L'invention concerne la caractérisation et l'utilisation de promoteurs viraux puissants pour l'expression de gènes dans des bactéries et des champignons, en particulier dans des levures. L'invention est basée sur le fait qu'on a constaté, de façon surprenante, que des CFDV-ADN (virus ADN de la pourriture de la feuille de cocotier) et des fragments de CFDV-ADN contenaient une région active en tant que promoteur, également dans des bactéries et des champignons, en particulier des levures, bien qu'ils proviennent d'un virus infectant les plantes monocotylédones. L'activité des promoteurs spécifiés dans E. coli est notablement plus élevée que celle du promoteur CaMV 35S également actif dans des bactéries.

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