A method for producing preparations of mature and immature...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

IPC codes

C12N 5/071 (2010.01) A61K 35/39 (2006.01) A61P 3/00 (2006.01) A61P 3/10 (2006.01) C12N 5/00 (2006.01)

Type

Patent

Patent number

CA 2363971

Description

The invention relates to a method for preparing a preparation of mammalian pancreatic endocrine cells comprising the steps of dissociating intact pancreatic tissue into a cell suspension comprising single cells and cell aggregates and of enriching said cell suspension with regard to the content of endocrine cells to a preparation of viable endocrine cells obtainable by this method and to its use in the treatment of diabetes mellitus, preferably in humans, and in diagnostic procedures.

L'invention porte sur une méthode permettant de produire une préparation de cellules endocrines du pancréas d'un mammifère selon les étapes suivantes : dissociation d'un tissu intact de pancréas pour préparer une suspension de cellules contenant des cellules uniques et des agrégats de cellules et enrichissement du contenu en cellules endocrines de ladite suspension. L'invention porte aussi sur une préparation de cellules endocrines viables par cette méthode et sur l'utilisation de cette préparation dans le traitement du diabète, de préférence chez l'humain, et dans des méthodes diagnostiques.

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