Novel hiv-1 reverse transcriptase codon deletion and its use...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/54 (2006.01) A61K 31/7076 (2006.01) C12N 9/12 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2684061

The present invention provides an isolated HIV-1 mutant and isolated nucleic acid molecules comprising HIV-RT coding sequences harboring a novel mutation in the S68 codon, and in particular, deletions of the S68 codon. This novel deletion reduces the sensitivity of HIV to various nucleoside reverse transcriptase inhibitors. Methods of using this mutation for selecting effective antiretroviral agents in vitro and in vivo, methods for monitoring infection progression in HIV-infected individuals and methods for avoiding the emergence of and/or to treat individuals infected with HIV comprising mutations at the S68 codon of HIV-RT, e.g., S68del, are provided.

La présente invention concerne un mutant isolé de VIH-1 et des molécules d'acide nucléique isolées comprenant des séquences codant pour la transcriptase inverse de VIH abritant une nouvelle mutation du codon S68, et en particulier, des délétions du codon S68. Cette nouvelle délétion réduit la sensibilité du VIH à divers inhibiteurs de la transcriptase inverse de nucléoside. L'invention concerne des procédés d'utilisation de cette mutation permettant de choisir des agents anti-rétroviraux efficaces in vitro et in vivo, des procédés de surveillance de l'évolution de l'infection chez les sujets infectés par le VIH et des procédés permettant d'éviter l'émergence d'une infection à VIH ou de traiter les individus infectés par le VIH comprenant des mutations au niveau du codon S68 de la transcriptase inverse de VIH, par exemple, S68del.

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