Microbial inhibition test kit and method

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/18 (2006.01) C12M 1/34 (2006.01)

Patent

CA 2056581

ABSTRACT OF THE DISCLOSURE A test kit and method for the detection of antimicrobial drugs at low concentrations in a sample in a test container, such as milk, which includes a Bacillus stearothermophilus (BST) tablet and a medium tablet with nutrients to stress the BST and, optionally, a pH indicator. The method comprises preheating a test sample to destroy natural inhibitors, cooling the sample, adding a BST tablet, rapidly heating to effect synchronization of BST germination, adding a medium tablet, incubating and detecting the presence or absence of the antimicrobial drug. The test apparatus includes optionally a test apparatus for the controlled, sequenced heating and cooling of the test sample.

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