Method for the detection of lysozyme using a protein error...

Details Method for the detection of lysozyme using a protein error... Method for the detection of lysozyme using a protein error...

C - Chemistry, Metallurgy

12

Q

C12Q 1/34 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2214400

The determination of lysozyme in urine is carried out by contacting the urine with a reagent system containing a buffer and a protein error indicator dye. Other proteins normally found in urine can compete with the lysozyme for interaction with the protein error indicator thereby affecting the specificity of the test for lysozyme. The present invention involves the addition of certain alkyl sulfonic acids or sulfonates to increase both specificity and sensitivity of the reagent for lysozyme.

Détection de lysozyme dans l'urine par contact de l'urine avec un système de réactifs contenant un tampon et un colorant indicateur d'erreur sur l'identité de la protéine. D'autres protéines habituellement présentes dans l'urine peuvent livrer concurrence au lysozyme pour interagir avec l'indicateur d'erreur, nuisant ainsi à la spécificité du test de détection de lysozyme. L'invention concerne l'addition d'acides alcoylsulfoniques ou d'alcoylsulfonates pour accroître à la fois la spécificité et la sensibilité du réactif à l'égard du lysozyme.

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