Proliferative action of leukaemia inhibitory factor on...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/06 (2006.01) A61K 35/12 (2006.01) A61K 38/19 (2006.01) A61K 38/20 (2006.01) C12N 5/08 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2045630

(57) Abstract The present invention relates to the use of leukaemia inhibitory factor (LiF), alone or in combination witj other cytokines such as interleukin-6 (IL-6) and/or transforming growth factor .alpha.(FGF.alpha.) and/or fibroblast growth factor (FGF), to stimulate the ?liferation and/or differentiation of mammalian satellite cells. THe present invention also contemplates a method comprising ?yoblast transfer therapy whereby LIF, alone or in combination with other cytokines, is/are employed to proliferate and/or dif- ferentiate mammalian satellite cells into myoblasts. The present invention is also directed to a cell activating composition and a pharmaceutical composition comprising LIF alone or in combination with other cytokines to promote proliferation and/or differ- entiation of mammalian satellite cells in vitro and in vivo, respectively.

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