Stress tolerance in plants through selective inhibition of...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01) A01H 3/00 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12N 15/29 (2006.01) C12N 15/55 (2006.01) C12N 9/12 (2006.01) C12N 9/16 (2006.01)

Patent

CA 2588372

The present invention relates to transgenic plants comprising an isolated DNA molecule comprising a polynucleotide that encodes a nucleic acid that down- regulates an endogenous T6PP gene, wherein the polynucleotide is under the control of a promoter that is stress-inducible and is expressed predominantly in vegetative tissue. The promoter may also be developmentally expressed in maturing kernels. Expression of the polynucleotide results in the increased availability of carbon to developing florets/kernels when plants are subject to environmental stress, such as a water deficit. The DNA molecule of the invention thereby permits more photosynthate to be directed to the developing ovules/embryos resulting in stabilized yield in growing environments that are subject to periodic stress.

L'invention concerne des plantes transgéniques comprenant une molécule d'ADN isolée qui contient un polynucléotide codant pour un acide nucléique qui régule négativement un gène T6PP endogène. Le polynucléotide est sous le contrôle d'un promoteur inductible par le stress et exprimé principalement dans un tissu végétal. Ledit promoteur peut également être exprimé par développement dans des noyaux matures. L'expression du polynucléotide a pour résultat une augmentation de la disponibilité du carbone permettant de développer des fleurettes/noyaux lorsque les plantes sont soumises à un stress environnemental, tel qu'un manque d'eau. La molécule d'ADN de l'invention permet de ce fait d'orienter plus de photosyntate vers le développement d'ovules/embryons, ce qui a pour résultat un rendement stabilisé dans des environnements de croissance soumis à un stress périodique.

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